Publications논문

Effect of C-60 Fullerene on the Duplex Formation of i-Motif DNA with Complementary DNA in Solution
2010-03-10 11:05:43 조회수1053
Kyeong Sik Jin, Su Ryon Shin, Byungcheol Ahn, Sang woo Jin, Yecheol Rho, Heesoo Kim, Seon Jeong Kim, Moonhor Ree Center for Bio-Artificial Muscle and Department of Biomedical Engineering, Hanyang University, Seoul 133-791, Korea 원문 링크 : http://seonjeongkim.cafe24.com/link

Abstract

The structural effects of fullerene on i-motif DNA were investigated by characterizing the structures of fullerene-free and fullerene-bound i-motif DNA, in the presence of cDNA and in solutions of varying pH, using circular dichroism and synchrotron small-angle X-ray scattering. To facilitate a direct structural comparison between the i-motif and duplex structures in response to pH stimulus, we developed atomic scale structural models for the duplex and i-motif DNA structures, and for the C60/i-motif DNA hybrid associated with the cDNA strand, assuming that the DNA strands are present in an ideal right-handed helical conformation. We found that fullerene shifted the pH-induced conformational transition between the i-motif and the duplex structure, possibly due to the hydrophobic interactions between the terminal fullerenes and between the terminal fullerenes and an internal TAA loop in the DNA strand. The hybrid structure showed a dramatic reduction in cyclic hysteresis.

 
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